The second Capto adhere step was performed in FT mode in both setups. DBC of Capto S ImpAct at different ionic strengths and pH values Buffer pH NaCl (mM) DBC (mg/mL) 50 mM sodium acetate 5.0 71 50 mM sodium acetate 5.0 50 109 50 mM sodium acetate 5.5 50 122 100 mM sodium acetate 6.0 124 50 mM sodium acetate 6.0 89 Buffer capacity was too weak to add 50 mM NaCl at pH 6. Two setups were evaluated, where the first Capto S ImpAct polishing step was run in either bind-elute (B/E) or flow-through (FT) mode. Capto™ S ImpAct and Capto adhere media in serially connected columns with an intermediate in-line conditioning step were used. The capture step was followed by two polishing steps in an STP setup on the ÄKTA pure chromatography system. Using the 3C PCC setup, the capacity utilization could be increased by 56% as compared with an equivalent batch run. viernes santo 2022 malaga Capto S ImpAct, 10 L Cytiva WebSHOCKWAVE Impact Duty 1Drive 33MM Standard 6 Point Socket 49-66-6598. The results showed consistent yield and purity over time. To assess robustness of the setup, 10 cycles were performed. MAb capture was performed using MabSelect SuRe™ LX protein A chromatography medium (resin) in a three-column PCC (3C PCC) setup on the ÄKTA™ pcc 75 chromatography system. PreDictor RoboColumn Capto S ImpAct, 600 L units were used for fast determination of DBC using a design of experiments (DoE) approach. Furthermore, the small bead size enables high-resolution purifications. The polymeric ligand, in combination with the attributes of the base matrix, gives this cation exchanger a high binding capacity. In this study, periodic counter current (PCC) chromatography and straight-through processing (STP) technologies were evaluated in a continuous three-step monoclonal antibody (MAb) purification process. This chromatography resin is part of a platform of high-resolution resins based on the Capto product line.
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